Amplifying Lambda DNA
Topic: Viruses
Organism: None
Grade Level: Middle/high school
Organism: None
Grade Level: Middle/high school
Learn about PCR by amplifying a sequence from the bacteriophage Lambda genome then verifying the size of the amplified product by comparing it to DNA size markers.
Essential Equipment (in addition to standard lab supplies):
● Electrophoresis supplies (camera, chamber, power supplies, and transilluminator)
● Microcentrifuge
● Micropipets (1–1000 uL)
● Thermocycler
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students