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Bacterial Transformation
Topic: Transformation
Organism: Bacteria
Grade Level: Middle/high school Middle/high school
Genetically engineer bacterial cells with the pGFP plasmid to resist ampicilin and glow green.
Essential Equipment (in addition to standard lab supplies):
● Water bath (42°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students
Protocol variations are listed below
Version: Bacterial Transformation: pAMP
Organism: Bacteria
Grade Level: High School
Genetically engineer bacterial cells using the pAMP plasmid to resist ampicilin.
Essential Equipment (in addition to standard lab supplies):
● Water bath (42°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students
Version: Bacterial Transformation: pBLU
Organism: Bacteria
Grade Level: High School
Genetically engineer bacterial cells using the pBLU plasmid to resist ampicilin and tolerate lactose.
Essential Equipment (in addition to standard lab supplies):
● Water bath (42°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students
Version: Bacterial Transformation: pVIB
Organism: Bacteria
Grade Level: High School
Genetically engineer bacterial cells using the pVIB plasmid to resist ampicilin and glow in the dark.
Essential Equipment (in addition to standard lab supplies):
● Water bath (42°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students
Version: Bacterial Transformation: pAMP/KAN
Organism: Bacteria
Grade Level: High School
Genetically engineer bacterial cells using the pAMP/KAN plasmid to resist ampicilin and kanamycin.
Essential Equipment (in addition to standard lab supplies):
● Water bath (42°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students
Version: Bacterial Transformation: Glowing Genes
Organism: Bacteria
Grade Level: Middle School
Genetically engineer bacterial cells with the pGFP plasmid to resist ampicilin and glow green. Shortened version for middle school.
Essential Equipment (in addition to standard lab supplies):
● Water bath (42°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students