Constructing an RNAi Feeding Vector
Topic: RNAi
Organism: Animal
Grade Level: Middle/high school
Organism: Animal
Grade Level: Middle/high school
Create a gene-specific RNAi plasmid and transform bacteria to be used as the feeding strain to silence a gene of interest in C. elegans.
Essential Equipment (in addition to standard lab supplies):
● Electrophoresis supplies (camera, chamber, power supplies, and transilluminator)
● Microcentrifuge
● Micropipets (1–1000 uL)
● Stereomicroscope (min 30x) with transillumination
● Thermal cycler
● Water bath (37°C and 42°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students