Detecting Epigenetic DNA Methylation in Arabidopsis
Topic: Epigenetics
Organism: Plant
Grade Level: Middle/high school
Expore the epigenetic effect of DNA methylation of a gene.
Essential Equipment (in addition to standard lab supplies):
● Electrophoresis supplies (camera, chamber, power supplies, and transilluminator)
● Microcentrifuge
● Micropipets (1–1000 uL)
● Thermal cycler
● Water bath or heat block (65°C and 37°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students