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Detecting a Transposon
Topic: Transposons
Organism: Plant
Grade Level: Middle/high school
Learn how transposable elements can be used to mutagenize and tag genes in corn (Zea mays or maize).
Essential Equipment (in addition to standard lab supplies):
● Electrophoresis supplies (camera, chamber, power supplies, and transilluminator)
● Microcentrifuge
● Micropipets (1–1000 uL)
● Thermal cycler
● Water bath or heat block (99°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students
Protocol variations are listed below
Version: Detecting a Transposon in Arapidopsis
Organism: Plant
Grade Level: High School
Learn how transposable elements can be used to mutagenize and tag genes in <i>Arabidopsis thaliana</i>.
Essential Equipment (in addition to standard lab supplies):
● Electrophoresis supplies (camera, chamber, power supplies, and transilluminator)
● Microcentrifuge
● Micropipets (1–1000 uL)
● Thermal cycler
● Water bath or heat block (99°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students