Examining the RNAi Mechanism
Topic: RNAi
Organism: Animal
Grade Level: Middle/high school
Organism: Animal
Grade Level: Middle/high school
Compare DNA mutation and dsRNA silencing to explore how RNAi disrupts the function of a gene.
Essential Equipment (in addition to standard lab supplies):
● Electrophoresis supplies (camera, chamber, power supplies, and transilluminator)
● Microcentrifuge
● Micropipets (1–1000 uL)
● Stereomicroscope (min 30x) with transillumination
● Thermal cycler
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students