Purification and Identification of Plasmid DNA
Topic: DNA Extraction + Restriction Analysis
Organism: Bacteria
Grade Level: Middle/high school
Organism: Bacteria
Grade Level: Middle/high school
Isolate DNA from cells and identify the molecular genotype using DNA restriction analysis.
Essential Equipment (in addition to standard lab supplies):
● Electrophoresis supplies (camera, chamber, power supplies, and transilluminator)
● Microcentrifuge
● Micropipets (1–1000 uL)
● Water bath or heat block (37°C)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students