Using E. coli Feeding Strains to Induce RNAi and Knock Down Genes
Topic: RNAi
Organism: Animal
Grade Level: Middle/high school
Organism: Animal
Grade Level: Middle/high school
Demonstrate how RNAi can be used to deduce the function of genes by feeding C. elegans with bacteria that will trigger the "silencing" of a gene and study the resulting altered phenotype.
Essential Equipment (in addition to standard lab supplies):
● Micropipets (10–1000 uL)
● Stereomicroscopes (min 30x) with transillumination
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students