Protein Purification
Topic: Proteins
Organism: Bacteria
Grade Level: Middle/high school
Organism: Bacteria
Grade Level: Middle/high school
Isolate the green fluorescent protein (GFP) from E.coli cells.
Essential Equipment (in addition to standard lab supplies):
● Microcentrifuge
● Micropipets (10–1000 uL)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students
Protocol variations are listed below
Version: Protient Purification: pAMP
Organism: Bacteria
Grade Level: High School
Organism: Bacteria
Grade Level: High School
Isolate the ampicilin antiobiotic resistance protein from <i>E.coli</i> cells.
Essential Equipment (in addition to standard lab supplies):
● Microcentrifuge
● Micropipets (10–1000 uL)
Resources:
Protocol Information
Preparation time:
Minimal
Involved
Equipment required:
Common
Specialized
Conceptual difficulty:
Easy
Challenging
Technical difficulty:
Easy
Challenging
Teachers
Students